• About Site Author
• More Tutorials
• Toolbar
• Life Science Jobs

Proteomics Databases

• SWISS-2DPAGE: Two-dimensional polyacrylamide gel electrophoresis database
• Rat Serum Protein Study Group
• Parasite Proteome Maps
• Rice Proteome Database
• Rice 2D Map of anther
• COMPLUYEAST-2DPAGE
• SIENA-2DPAGE Database
• PMMA-2DPAGE: Purkyne Military Medical Academy 2D-PAGE database
• Joint Proteomics Laboratory: JPSL Proteomic Databases
• Yeast Protein Complex Database
• 2D Gel Map of Saccharomyces cerevisiae (Yeast) proteins
• HumAN 2D PAGE Databases for Proteome Analyses in Health and Diseases
• MitoDat: A database of mitochondrial ptroteins encoded by nuclear genes
• DIP: Database of Interacting Proteins
• Enzyme nomenclature database at Expasy
• PROSITE: Database of protein domains, families and functional sites
• Proteome Database System for Microbial Research at Max Planck Institute for Infection Biology
• Phospho.ELM database: Protein phosphorylation Database
• OGP-WWW: Two-dimensional polyacrylamide gel electrophoresis database
• HSC-2DPAGE: 2-DE Gel Protein Databases at Harefield
• Global Proteome Machine Database at Rockfeller University

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)

• A good introductory information on SDS-PAGE
• SDS-PAGE by Ed Rybicki and Maud Purves, University of Cape Town
• Protein Gel Electrophoresis. By David R. Caprette, Rice University
• A good animation tutoria to learn SDS-PAGE
• HOW DOES AN SDS PAGE GEL WORK. By Dyche Mullins
• A step-by-step SDS-PAGE Protocol
• PAGE --- recipe calculator
• A good SDS-PAGE and Western Blotting Protocol
• Another good Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE) protocol
• A Rapid Silver staining Method: Developed by N.Sivaramaiah and krishan Mohan, Birla Institute of Scientific Research, Jaipur, India.
• SDS-PAGE: Recipe and protocol at Mullins Molecular Retrovirolgy Lab
• A brief SDS-PAGE and Western Blot Protocol
• Another PROTOCOL FOR SDS-PAGE
• Here's another for SDS-PAGE and Western blot
• Electrophoresis Principles expalined
• An excellent SDS-PAGE troubleshooting guide by David R. Caprette

Two-Dimensional PAGE (2D-PAGE)

• 2D Gel Electrophoresis Tutorial
• A detailed 2D PAGE tutorial by James Jefferies
• 2D PAGE protocol at ExPASy
• An excellent detailed laboratory manual on 2D PAGE by Prof. Dr. Angelika Gorg
• A good article on two-dimensional polyacrylamide gel electrophoresis by Richard Twyman
• 2D PAGE at APAF (Australian Proteome Analysis Facility Ltd )
• Isoelfocus 3.0: A multimedia computer-aided learning program on Isoelectric Focusing and its derived techniques

Mass Spectrometry

• An Introduction to Mass Spectrometry. By Dr Alison E. Ashcroft
• Introduction to Mass Spectrometry (MS)
• MS tutorial by Waters.com
• Mass Spectrometry: A tutorial
• MASS SPECTRA - THE MOLECULAR ION (M+) PEAK
• Instrumentation and Ionization Methods Tutorial: Maintained by Department of Chemistry, Washington University
• Mass Spectrometry by Richard Caprioli and Marc Sutter, revised by Markus Stoeckli
• Time Of Flight / TOF Mass Spectrometer Tutorial
• MALDI-TOF Mass Spectrometry: A tutorial by Dr. Ken Mauritz
• Protein Identification Tutorial at IonSource
• Isotopes and Mass spectrometry: an IonSource.Com tutorial
• What is Electrospray?
  An introduction to electrospray ionisation (ESI)
• Principles of MS Quantitation: at IonSource
• MALDI Tutorial: Maintained by Department of Chemistry, Washington University
• Mass Spectometry Tutorial Lectures by American Society for Mass Spectrometry: Require Macromedia Flash Player

Gel Analysis Software

• TotalLab: Analysis of 1D gel images
• Progenesis: 2D Gel Analysis
• IMAGEMASTER 2D PLATINUM & MELANIE: 2D GEL ANALYSIS
• PDQuest Software: A software tool for comparing 2D gels
• GELLAB-II: 2D Gel Analysis Software
• NCI Flicker Web Server: Compare images across the Internet
• The Make2D-DB II Package: A software to create and keep up-to-date 2-DE databases (an open source software)
• Compute pI/Mw tool at ExPASy: A tool for the computation of the theoretical pI (isoelectric point) and Mw (molecular weight) of a protein

MS Analysis Software

• GPMAW - General Protein/Mass Analysis for Windows
• MSight: A software tool for the representation of mass spectra
• Popitam: protein characterization using tandem mass spectrometry data
• Phenyx: A software platform for the identification and characterization of proteins and peptides from mass spectrometry data
• Insilicos Viewer Version 1.4.5: A program for viewing tandem mass spec proteomics data